Austin Pathology

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Immunophenotyping - Flow Cytometry

Alternate Names
Cell Surface Markers, CLL, Immunophenotype, Leukaemia Panel, Leukaemia Screen, Lymphoma, Lymphoproliferative Screen, NHL, Mantle Cell Lymphoma, Myeloma
Ordering Information

REQUEST FLOW CYTOMETRY ON TISSUE FOR:

  Suspected lymphoma (past history of lymphoma, lymphadenopathy, radiology/imaging suggests lymphoma)

    Flow is of no value in Hodgkin lymphomas

TISSUE SAMPLE REQUIREMENTS

  Separate fresh tissue specimen in sterile pot or in tissue preservative (RPMI, pink liquid – preferred if sending after hours)

  Label as urgent

  If uncertain, please discuss with Anatomical Pathologist

PLEASE NOTE: Anatomical pathology cannot/will not divide smaller specimens such as core biopsies, these should have separate specimens for flow cytometry and histology.

We will only divide large specimens >1-2 cubic cm (e.g. whole lymph nodes, spleen)

For non lymph node tissue specimens that are small (often small and very important) ideally cases should be discussed with a pathologist before or during a procedure to ascertain the most appropriate distribution of tissue. An Anatomical Pathologist is available by telephone at all times.

Histology should usually be prioritized as this is more likely to be diagnostic.

 

Laboratory
Flow Cytometry
Specimen
Body Fluid
CSF
Tissue
See collection instructions
Bone Marrow
Lymph Node
Blood
Container
See collection instructions
Additional Collection Instructions

Blood:  DEDICATED 5 mL blood LITHIUM HEPARIN tube no gel, PLUS 4 mL blood EDTA tube.  Please do not combine with other tests using the same sample type, as separate tubes must be collected for these tests.

Bone Marrow:  LITHIUM HEPARIN or EDTA tube.  Separate tubes must be collected for the tests. Arrange with the Haematology Registrar, ph: 9496 5981.

Lymph Node:  Sterile container (no fixative) and send immediately to Anatomical Pathology.

Body Fluid:  Send immediately to Anatomical Pathology.

 

Instructions for: Metropolitan Collection Centres

  • DO NOT COLLECT AFTER MIDDAY FRIDAY OR AT ALL ON SATURDAY.
  • DO NOT COLLECT AFTER MIDDAY THE DAY BEFORE A PUBLIC HOLIDAY.

Instructions for: Regional Collection Centres

  • DO NOT COLLECT ON FRIDAY OR SATURDAY.
  • DO NOT COLLECT THE DAY BEFORE A PUBLIC HOLIDAY.
Minimum Adult Volume
None
Minimum Paediatric Volume
None
Notes

On Fridays and prior to public holidays, specimens must be received in Pathology before 3pm and marked as URGENT.

Frequency
Monday - Friday
Test Code
FLOW
Container ID
CH, CHS
Storage Instructions
Store specimen at room temperature
Transport Instructions
Transport at room temperature
Additional Transport Instruction

Instructions for: Regional Specimen Receptions

  • DO NOT SPIN. KEEP AT ROOM TEMPERATURE.
  • Forward sample to the Heidelberg Laboratory, Flow Cytometry.

 

Instructions for: Heidelberg Specimen Reception

  • DO NOT SPIN. KEEP AT ROOM TEMPERATURE.
  • If a sample is collected outside of the specified collection timeframe, forward the sample to Flow Cytometry.
Laboratory Notes

Instructions for: Metropolitan & Regional Specimen Receptions

Request for Flow Cytometry received without any clinical notes will require further clarification from the requesting doctor.

This test (Immunophenotyping - FLOW) is used for oncology patients with suspected malignancies. 

Other Flow Cytometry tests can be found in the table below:

Test Code Description
Immunophenotyping FLOW Diagnosis of patients with suspected malignancy.
Lymphocyte Subsets SUBS Analysis of lymphocyte sub-populations
PNH Screen PNH Screening for Paroxysmal Nocturnal Haemoglobinuria
CD34 Enumeration PRE34 Enumeration of CD34+ stem cells for patient undergoing a stem cell harvest by apheresis.
HLA B27 Screen HLAB27 Screening of the HLA B27 antigen.
MRD Analysis MRD Minimal residual disease monitoring for patients with known leukaemia.

Refer to each test for specimen and handling requirements. Contact Flow Cytometry (9496 5909) for assistance with ambiguous requests.